Journal: Scientific Reports

Article Title: Identification of loci associated with susceptibility to bovine paratuberculosis and with the dysregulation of the MECOM , eEF1A2 , and U1 spliceosomal RNA expression

doi: 10.1038/s41598-020-79619-x

Figure Lengend Snippet: The MECOM expression is associated with the rs43744169. MECOM protein levels in plasmas from homozygous and heterozygous cows in the rs43744169 were measured with a bovine ELISA. Samples were selected from the case–control population and grouped according to the rs43744169 genotypes. Cows with the alleles T/C (N = 13) and C/C (N = 12) were associated with elevated MECOM protein levels when compared with the MECOM levels in the plasmas from cows with the two major alleles T/T (N = 11); P = 0.05 and P = 0.03, respectively. The median and the upper and lower quartiles of each group are represented by horizontal and vertical lines, respectively. The lowest point is the minimum of the data set and the highest point is the maximum of the data set. P-values were calculated using an unpaired t-test. *P-value ≤ 0.05. Each sample is represented by a circle on the dot plot.

Article Snippet: The levels of MECOM protein in plasma samples (50 μl) were assessed by using a quantitative sandwich ELISA according to the manufacturer’s instructions (MyBioSource, San Diego, US).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Control

Journal: Frontiers in Cellular Neuroscience

Article Title: Myelin Debris Stimulates NG2/CSPG4 Expression in Bone Marrow-Derived Macrophages in the Injured Spinal Cord

doi: 10.3389/fncel.2021.651827

Figure Lengend Snippet: NG2 expression at levels of the mRNA, intracellular protein, and secreted protein in BMDMΦ treated with myelin debris. (A) NG2 mRNA expression in BMDMΦ treated with myelin debris for 7 days. (B) NG2 protein expression in BMDMΦ treated with myelin debris for 7 days and 14 days assessed by WB images. Corresponding quantification of protein levels was determined by densitometry analysis relative to α-tubulin. The immunoblots were performed twice with similar results. C: control; T: myelin debris treatment. (C) NG2 protein expression in microglia with myelin debris for indicated time points assessed by WB images. Corresponding quantification of protein levels was determined by densitometry analysis relative to α-tubulin. The immunoblots were performed four times with similar results. C: control; T: myelin debris treatment. (D) Chondroitin sulfate proteoglycan 4 (CSPG4) in the supernatant of BMDMΦ treated with myelin debris for 7 days and 14 days detected by Enzyme-linked immunosorbent assay (ELISA). Data for all quantifications are shown as means ± SD of three separate BMDMΦ isolations from three separate mice ( n = 3). ** P ≤ 0.01.

Article Snippet: CSPG4 protein in BMDMΦ was quantified by a commercial quantitative sandwich ELISA kit (G-Biosciences Cat. #IT5412).

Techniques: Expressing, Western Blot, Control, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Physiology

Article Title: Combined Systemic Intake of K-ATP Opener (Nicorandil) and Mesenchymal Stem Cells Preconditioned With Nicorandil Alleviates Pancreatic Insufficiency in a Model of Bilateral Renal Ischemia/Reperfusion Injury

doi: 10.3389/fphys.2022.934597

Figure Lengend Snippet: Estimated levels of blood glucose, LDH, amylase, MPO, and TNF-α in all studied groups ( n = 6 in each group). p -value ≤0.05 is considered significant.

Article Snippet: Then using quantitative Sandwich Rat Cyclic Guanosine Monophosphate (c-GMP) ELISA Kit (MyBioSource, Catalog #MBS007871, the level of c-GMP was estimated and expressed per ml.

Techniques: